Figure 5.

Alb3.2 Interactions.

(A) Alb3.2 interacts with D1 and D2. Immunoprecipitations like those described for Figure 3 were performed with extracts from the strain containing FLAG-tagged Alb3.2 and, as controls, the wild type and the strain ΔD1-Alb3.2-FLAG (FuD7 lacking psbA transformed with Alb3.2-FLAG). Input refers to an immunoblot (IB) of the extract before immunoprecipitation (IP) with the antiserum indicated for the immunoblot.

(B) Alb3.2 interacts with PsaA and VIPP1. Similar immunoprecipitations of solubilized membranes of wild-type cells were performed with the indicated antisera (+Ab) or, as controls, with preimmune serum (−Ab). The input and flow through (f.t.) represent 10% of the proteins used for the immunoprecipitations. The immunoprecipitates were separated by PAGE and immunoblotted. Immunoblotting was performed with the indicated antisera.