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. 2000 Jan;11(1):39–50. doi: 10.1091/mbc.11.1.39

Figure 4.

Figure 4

In COS-7 cells incubated at 37°C, the effects of PDBu on the transport activity and the cell surface expression of Na,K-ATPase are dependent on PKCs and arachidonic acid metabolism. COS-7 cells expressing the wild-type Bufo α1 subunit were incubated for 30 min at 37°C in the absence (C) or presence of 10−7 M PDBu (P), 5 × 10−6 M arachidonic acid (A), 5 × 10−7 M GF109203X (a PKC inhibitor; G), GF109203X and PDBu (G+), 10−5 M oleoyloxyethylphosphocholine (a phospholipase A2 inhibitor; Ol), oleoyloxyethylphosphocholine and PDBu (Ol+); 17-octadecynoic acid (a cytochrome P450 inhibitor; Od), and 17-octadecynoic acid and PDBu (Od+). (A) Exogenous Na,K-ATPase-mediated 86Rb uptake was measured in the presence of 2.5 × 10−6 M ouabain under initial rates of influx. Results are expressed as fractional change (with respect to control value) and are means ± SE from 6–14 independent experiments (**, p < 0.01). (B) After streptavidin precipitation, the cell surface–expressed Bufo α1 subunits were detected by immunoblotting using a specific anti-Bufo α1 subunit antibody. Results are expressed as fractional change (with respect to control values) and are means ± SE from six or seven independent experiments (*, p < 0.05).