Table 1.
List of proteins on the 2-DE map of Halobacterium NRC-1, identified byMALDI-TOF and LC-MS/MS
| No. | ORF No. | Protein Name | MS/MASCOT Score | Mowse Score | % Coverage | Annotation | Putative kDa/pI | Calculated kDa/pI |
| 1 | 2338 | polA2* | 15 | 39 | 13 | DNA polymerase | 150/4.38 | 148.9/4.39 |
| 2 | 2096 | cctB | 668 | 33 | Thermosome β | 70/4.12 | 69/4.1 | |
| 3 | 2381 | uvrC* | 40 | 51 | 18 | Excision nuclease chain C | 63.7/4.8 | 63/4.89 |
| 4 | 6363 | Orc4/CDC6 | 82 | 30 | Orc4/CDC6 | 58/4.51 | 58/4.52 | |
| 5 | 715 | thiC | 57 | 28 | Thiamine biosynthesis protein | 51/4.15 | 51.5/4.15 | |
| 6 | 1601 | gcvP2* | 20 | 47 | 20 | Glycine Dehydrogenase II | 50/4.43 | 51/4.4 |
| 7 | 1356 | fumC | 378 | 27 | Fumarate hydratase | 50/4.4 | 50/4.38 | |
| 8 | 2649 | eef1A | 158 | 18 | Translation elongation factor α subunit | 46/4.32 | 46.2/4.3 | |
| 9 | 2606 | thiD | 83 | 21 | Thiamine biosynthesis protein | 45/4.2 | 47/4.17 | |
| 10 | 95 | gapB | 220 | 13 | Glyceraldehyde 3-PDH | 35/4.26 | 35.5/4.2 | |
| 11 | 903 | yufN* | 20 | 47 | 8 | ABC transporter | 33/4.02 | 33.2/4.02 |
| 12 | 683 | vng0683C* | 73 | 63 | 14 | Hypothetical | 28/4.69 | 28.9/4.6 |
| 13 | 2331 | vng0309C* | 56 | 46 | 12 | Hypothetical | 25/4.48 | 26.9/4.31 |
| 14 | 1027 | tpiA | 50 | 13 | Triosephosphate isomerase | 21/3.97 | 21.3/4.01 | |
| 15 | 2443 | dpsA | 60 | 19 | Stress induced DNA binding protein | 20/4.14 | 20.4/4.16 | |
| 16 | 890 | imD2 | 196 | 25 | Inosine-5'-monophosphate dehydrogenase | 20/4.76 | 20.1/4.8 | |
| 17 | 1914 | ppiA* | 20 | 45 | 11 | Peptidyl proryl isomerase | 20/4.02 | 21/4.1 |
| 18 | 633 | purE | 72 | 20 | Phosphoribosylamino subunit | 21/4 | 21.7/4.07 | |
| 19 | 1180 | msrA | 71 | 38 | Peptide methionine sulfoxide red. | 19.4/4.2 | 21.1/4.18 | |
| 20 | 1607 | cheC2 | 76 | 12 | Chemotaxis protein | 21/4.23 | 21.7/4.2 | |
| 21 | 2118 | pyrE2* | 25 | 51 | 22 | Orotatephosphoribosyl transferase | 18/4.11 | 19.2/4.13 |
| 22 | 626 | maoC2* | 27 | 52 | 22 | Acyl Dehydratase | 16.8/4.15 | 16.9/4.2 |
| 23 | 1807 | vng1807H | 55 | 38 | Hypothetical | 15/4.29 | 16/4.32 | |
| 24 | 6254 | vng6254* | 21 | 42 | 12 | Conserved Hypothetical | 11.4/3.85 | 12.2/3.9 |
| 25 | 1137 | rpl18E* | 22 | 44 | 17 | Ribosome assembly | 12.5/5.6 | 12.1/5.62 |
| 26 | 1157 | rphs6* | 30 | 47 | 12 | Ribosome assembly | 12.7/3.79 | 12.5/3.81 |
| 27 | 2467 | rpl31E | 110 | 71 | Ribosome assembly | 10/4.52 | 10.2/4.4 | |
| 28 | 1922 | trh5* | 32 | 54 | 26 | Lrp like regulator | 8.0/4.24 | 8.4/4.11 |
| 29 | 1300 | vng1300H* | 49 | 71 | 91 | Hypothetical | 6.5/3.91 | 8.0/4.0 |
| 30 | 2047 | rps27aE* | 30 | 49 | 10 | Ribosome assembly | 6/3.87 | 8.2/3.86 |
*Proteins identified by MALDI-TOF