Figure 1.

c-Yes and p120^CTN translocate to cell–cell adhesions in epithelial cells in response to high Ca²⁺. Shown are confocal immunofluorescence micrographs of primary human epidermal keratinocytes, HEKs (A–E), and SCC-13 (F and G) cells stained with anti-c-Yes (A, B, C, F, and G) or anti-p120^CTN (D and E). HEKs were maintained in low Ca²⁺ (A and D) or transferred to high Ca²⁺ for 24 h (B, C, and E). SCC-13 cells were transferred to low-Ca²⁺–containing medium for 24 h (F) and were subsequently transferred to high Ca²⁺ for 24 h (G). Cytochalasin D was added to HEKs at a concentration of 5 μmg/ml as they were transferred to high Ca²⁺ (C). Bars, 25 μm.