Figure 3.

Induction of a kinase-defective (KD) c-Src protein also induces cell–cell contact and E-cadherin redistribution. (A) Immunoblot using avian-specific mAb EC10 to detect only exogenous c-Src-KD expression in SCC 13 parental cells and SCC-13/ecSrc-KD transfectant cells that were untreated (uninduced), treated with ethanol alone (control), or treated with 10 μM muristerone A for 24 h (upper panel). Both endogenous and exogneous Src expression were monitored by immunoblotting with anti-Src (mAb 327) that reacts with both human and avian Src (lower panel), (B) Phase-contrast images; (C) E-cadherin confocal immunofluorescence micrographs of SCC-13/ecSrc-KD cells maintained in low Ca²⁺ and untreated (left panels) or treated with 10 μM muristerone A for 24 h (right panels). Bars, (B) 200 μm; (C) 25 μm.