Figure 7.

Constitutively active mutants of the Rho GTPases relocalize CEACAM1-L at cell–cell boundaries in Swiss 3T3 Cells. Quiescent serum-starved Swiss 3T3 fibroblasts were injected with a pXM139 vector expressing CEACAM1-L (A–F) or CEACAM1-S (G and H) and a pRK5 vector encoding either the myc-tagged constitutively activated L61Cdc42 protein (A and B, G and H), the L61Rac1 protein (C and D), or the L63RhoA protein (E and F). After 12 h of expression, permeabilized cells were stained for CEACAM1-L expression using the 231 anti-CEACAM1 polyclonal antibody and an FITC-conjugated anti-rabbit antibody (A, C, E, and G). Actin was detected using TRITC-conjugated phalloidin (B, D, F, and H).