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. Author manuscript; available in PMC: 2006 Jun 8.
Published in final edited form as: Nat Cell Biol. 2000 Mar;2(3):163–167. doi: 10.1038/35004038

Figure 3.

Figure 3

PACS-1 binds to Nef in an acidic-cluster-dependent manner. a, Top, acidic-cluster-dependent co-immunoprecipitation of PACS-1 and Nef (wild-type or acidic-cluster-mutated) in expressing cells. Centre, the relative amount of co-immunoprecipitated (CO-IP) PACS-1ha. Bottom, levels of Nef and PACS-1ha in the input before co-immunoprecipitation. b, HtPACS-1fbr interacts with GST–Nef, but only weakly with GST–Nef EEEE65AAAA and with GST. Proteins bound to GST or conjugates were analysed by western blotting with antiserum 678 (anti-PACS-1fbr). GST–Nef maximally bound 17% of input HtPACS-1fbr. Assays were performed in triplicate. Error bars represent s.d.Values on the ordinate in a, b are arbitrary units, the reference (empty vector in a, GST in b) being assigned a value of 1.