Figure 2.

Effects of NO^• and the p38 MAPK inhibitor SB202190 (SB) on MAPK phosphorylation. (A) NO^• increases p38 MAPK phosphorylation, an effect blocked by SB (0.1 µM). (B) NO^• increases Erk1/2 phosphorylation, an effect enhanced by SB (0.1 µM). (C) SB (0–5 µM) alone increases Erk1/2 phosphorylation. THP-1 cells (1 × 10⁷) were stimulated with LPS (1 µg/ml) for 4 h. After 30 min treatment with ActD (2.5 µg/ml) in the absence (control) or presence of SB, cells were incubated without or with GSNO (0–800 µM) for another 30 min, as indicated and then lysed. Each experiment was repeated at least twice with similar results.