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Characterization of the interaction between CSA and CSB. (A) Immunoblot detection of CSB protein in Flag-CSA (lanes 1,2) and Flag-DDB2 (lanes 3,4) complexes immunoprecipitated (IP) with anti-Flag antibodies from HeLa cell extract. (B) Glycerol gradient sedimentation analysis of the Flag-CSA complex. Immunoblot analysis of the distribution of CSB, CSN, and Cul4A in fractions of the gradient.
