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. 2006 Jun 1;20(11):1441–1446. doi: 10.1101/gad.378706

Figure 5.

Figure 5.

Early insulin expression is independent of XPtf1a/p48, while later pancreatic insulin and glucagon expression depends on XPtf1a/p48. (A) Knockdown of XPtf1a/p48 by antisense MO injection results in a loss of exocrine pancreatic gene expression, while early dorsal insulin expression remains unaffected. (Panels 1,3) Transverse vibratome sections (panel 1, 100%, n = 85; morphometric quantification of insulin expression, average pixel number in panel 1, 12,265 ± 5152; panel 3, 15,537 ± 2108). (Panels 2,4) Ventral view, head toward the top (panel 2, 77%, n = 27). (B) The second phase of endocrine pancreatic differentiation is blocked upon knockdown of XPtf1a/p48 by antisense MO injection. Immunohistochemical analysis of insulin and glucagon expression in uninjected control (panels 3,4) and in XPtf1a/p48 MO-injected embryos (panels 1,2) at stage 48 of development. Phenotype in panels 1 and 2, 60%, n = 15. Bar, 200 μm.