Figure 2.

MTF-1, TFIID, and MED are recruited to the MtnA gene. (A–C) ChIP performed as described in the Materials and Methods section. Polyclonal antibodies used for immunoprecipitation are indicated below each column. Preimmune sera, normal rabbit IgG (RIgG), and normal guinea pig IgG (GIgG) serve as negative controls. White bars represent results from untreated S2 cells, and black bars represent results from S2 cells treated with copper for 6 h at 25°C. A diagram of the region amplified and the position of the primers used are shown below the graph.