Figure 4.

Recruitment of coactivators to the transgenic locus. (A–E) In situ staining to determine protein recruitment to the transgenic cluster. The protein specified above the first column in each panel was detected with an FITC conjugated secondary antibody. In the middle column of each panel, MTF-1 was detected with a Rhodamin-X conjugated secondary antibody, and DNA was stained with DAPI. The third column shows the merge of these channels. In each panel, the top row shows a cell in the absence of copper; the bottom row shows a cell in the presence of copper for 2 h (A,B,D,E) or 4 h (C). Scale bar, 2 μm.