Figure 7.

Depletion of TFIID suppresses depletion of MED. (A) Western analysis of nuclear extracts of S2 cells treated with dsRNA directed against either TAF4 (TFIID) or MED17, or both. Nontreated S2 cell extracts were serially diluted to provide an estimate of the depletion efficiency. Positions of TAF4 and MED17 are indicated by arrows. The asterisk denotes a cross-reacting band in the anti-MED17 sera. (B) Primer extension analysis (as in Fig. 3A) of S2 cells treated with dsRNA against the subunits or combinations of subunits indicated above each lane. (C) Quantitative PCR analysis of MtnA transcripts (as in Fig. 3B) of S2 cells treated with dsRNA against the subunits or combinations of subunits indicated below each column.