Fluorescence photographs of fractalkine-IR (red) in a vascular bed and PGP 9.5-IR nerve fibers (green) in the suburothelial plexus in whole mount preparations of the urothelium/lamina propria (A) or detrusor smooth muscle (B). Intense fractalkine-IR was present in the vascular bed in the urinary bladder in all experimental groups examined (A, control; B, 4 hr CYP-treatment). Extensive PGP 9.5-IR nerve fibers were present abundantly in the urothelium/lamina propria (A, green) and were more diffuse in the in detrusor smooth muscle whole mount (B, arrows). In no instance, was fractalkine-IR colocalized with PGP9.5-IR nerve fibers in the suburothelial plexus but PGP 9.5 nerve fibers were observed in close proximity to the fractalkine-IR vascular bed (A, arrows). Calibration bar represent 80 μm.