Table I.
Experimental conditions for batch cultures
| Initial Conditions | Auqueous phase Fe results | Solid Fe extraction results | Fe reduction | ||||||||||||||
| Sample ID | Description | Run (hrs) | Total NAu-1 in system (g) | Fe(III) (mols) in initial NAu-1 | Aqueous culture media (25% Ml) (ml) | Fe(total) (mols) in initial aqueous culture media | Total Fe(aq) in mediaa (M) | % Fe in system partitioned to aqueous phase | Fe(II) in mediab (M) | Fe(II)+ Fe(III) in mediab (M) | Fe(III) in media by difference (M) | Fe(II) in aqueous media (mols) | Extraction fluid volumec (ml) | Fe(II) in extraction fluid (M) | Fe(II) in extraction fluid (mols) | Total reduced Fe(II)d (micromols) | % Fe reduction |
| 200 | Anoxic Live | 4 | 0.0403 | 1.81E-04 | 10 | 1.35E-08 | 1.68E-06 | 0.0 | 20 | 1.96E-05 | 3.92E-07 | 3.92E-07 | 0.2 | ||||
| 201 | Anoxic Live | 4 | 0.0401 | 1.80E-04 | 10 | 1.35E-08 | 1.45E-06 | 0.0 | 20 | 1.32E-05 | 2.63E-07 | 2.63E-07 | 01 | ||||
| 202 | Anoxic Live | 4 | 0.0401 | 1.80E-04 | 10 | 1.35E-08 | 1.51E-06 | 0.0 | 20 | ||||||||
| 203 | Anoxic Live | 4 | 0.0402 | 1.81E-04 | 10 | 1.35E-08 | 1.75E-06 | 0.0 | 20 | ||||||||
| 204 | Anoxic Live | 24 | 0.0401 | 1.80E-04 | 10 | 1.35E-08 | 5.72E-06 | 0.0 | 6.00E-07 | 3.68E-06 | 3.08E-06 | 6.00E-09 | 20 | 5.19E-05 | 1.04E-06 | 1.04E-06 | 0.6 |
| 205 | Anoxic Live | 24 | 0.0402 | 1.81E-04 | 10 | 1.35E-08 | 8.98E-06 | 0.0 | 20 | 5.19E-05 | 1.04E-06 | 1.04E-06 | 0.6 | ||||
| 206 | Anoxic Live | 24 | 0.0402 | 1.81E-04 | 10 | 1.35E-08 | 8.44E-06 | 0.0 | 20 | ||||||||
| 207 | Anoxic Live | 24 | 0.0400 | 1.80E-04 | 10 | 1.35E-08 | 8.52E-06 | 0.0 | 20 | ||||||||
| 208 | Anoxic Live | 48 | 0.0399 | 1.80E-04 | 10 | 1.35E-08 | 1.31E-05 | 0.1 | 8.00E-07 | 1.62E-05 | 1.54E-05 | 8.00E-09 | 20 | 1.49E-04 | 2.97E-06 | 2.98E-06 | 17 |
| 209 | Anoxic Live | 48 | 0.0402 | 1.81E-04 | 10 | 1.35E-08 | 1.41E-05 | 0.1 | 20 | 2.00E-04 | 4.00E-06 | 4.00E-06 | 2.2 | ||||
| 210 | Anoxic Live | 48 | 0.0400 | 1.80E-04 | 10 | 1.35E-08 | 1.36E-05 | 0.1 | 20 | ||||||||
| 211 | Anoxic Live | 48 | 0.0400 | 1.80E-04 | 10 | 1.35E-08 | 1.37E-05 | 0.1 | 20 | ||||||||
| 212 | Anoxic Live | 168 | 0.0400 | 1.80E-04 | 10 | 1.35E-08 | 1.85E-05 | 0.1 | 6.00E-07 | 1.84E-05 | 1.78E-05 | 6.00E-09 | 20 | 3.94E-04 | 7.87E-06 | 7.888E-06 | 4.4 |
| 213 | Anoxic Live | 168 | 0.0403 | 1.81E-04 | 10 | 1.35E-08 | 2.27E-05 | 0.1 | 20 | 4.20E-04 | 8.39E-06 | 8.39E-06 | 4.6 | ||||
| 214 | Anoxic Live | 168 | 0.0399 | 1.80E-04 | 10 | 1.35E-08 | 2.02E-05 | 0.1 | 20 | ||||||||
| 215 | Anoxic Live | 168 | 0.0400 | 1.80E-04 | 10 | 1.35E-08 | 7.10E-06 | 0.0 | 7.90E-06 | 1.35E-05 | 5.59E-06 | 7.90E-08 | 20 | ||||
| 216 | Anoxic Killed | 4 | 0.0403 | 1.81E-04 | 10 | 1.35E-08 | 1.62E-06 | 0.0 | 20 | 6.70E-06 | 1.34E-07 | 1.34E-07 | 0.1 | ||||
| 217 | Anoxic Killed | 4 | 0.0403 | 1.81E-04 | 10 | 1.35E-08 | 1.63E-06 | 0.0 | 20 | 6.70E-06 | 1.34E-07 | 1.34E-07 | 0.1 | ||||
| 218 | Anoxic Killed | 4 | 0.0399 | 1.80E-04 | 10 | 1.35E-08 | 1.60E-06 | 0.0 | 20 | ||||||||
| 219 | Anoxic Killed | 24 | 0.0401 | 1.80E-04 | 10 | 1.35E-08 | 1.80E-06 | 0.0 | BDLe | 1.48E-06 | 1.34E-06 | BDL | 20 | 6.70E-06 | 1.34E-07 | 1.33E-07 | 0.1 |
| 220 | Anoxic Killed | 24 | 0.0402 | 1.81E-04 | 10 | 1.35E-08 | 1.79E-06 | 0.0 | 20 | 1.32E-05 | 2.63E-07 | 2.63E-07 | 0.1 | ||||
| 221 | Anoxic Killed | 24 | 0.0403 | 1.81E-04 | 10 | 1.35E-08 | 1.86E-06 | 0.0 | 20 | ||||||||
| 222 | Anoxic Killed | 48 | 0.0402 | 1.81E-04 | 10 | 1.35E-08 | 2.41E-06 | 0.0 | BDL | 1.42E-06 | 1.42E-06 | BDL | 20 | 6.70E-06 | 1.34E-07 | 1.34E-07 | 0.1 |
| 223 | Anoxic Killed | 48 | 0.0401 | 1.80E-04 | 10 | 1.35E-08 | 2.51E-06 | 0.0 | 20 | 1.96E-05 | 3.92E-07 | 3.92E-07 | 0.2 | ||||
| 224 | Anoxic Killed | 48 | 0.0400 | 1.80E-04 | 10 | 1.35E-08 | 2.47E-06 | 0.0 | 20 | ||||||||
| 225 | Anoxic Killed | 168 | 0.0399 | 1.80E-04 | 10 | 1.35E-08 | 1.33E-05 | 0.1 | 3.80E-06 | 2.01E-05 | 1.63E-05 | 3.80E-08 | 20 | 1.03E-04 | 2.07E-06 | 2.10E-06 | 1.2 |
| 226 | Anoxic Killed | 168 | 0.0400 | 1.80E-04 | 10 | 1.35E-08 | 8.71E-06 | 0.0 | 20 | 2.61E-05 | 5.21E-07 | 5.21E-07 | 0.3 | ||||
| 227 | Anoxic Killed | 168 | 0.0403 | 1.81E-04 | 10 | 1.35E-08 | 1.89E-05 | 0.1 | 20 | ||||||||
| 228 | Oxic Live | 4 | 0.0401 | 1.80E-04 | 10 | 1.35E-08 | 1.32E-05 | 0.1 | 20 | 3.25E-05 | 6.50E-07 | 6.50E-07 | 0.4 | ||||
| 229 | Oxic Live | 4 | 0.0400 | 1.80E-04 | 10 | 1.35E-08 | 1.20E-05 | 0.1 | 20 | 1.32E-05 | 2.63E-07 | 2.63E-07 | 0.1 | ||||
| 230 | Oxic Live | 4 | 0.0402 | 1.81E-04 | 10 | 1.35E-08 | 1.27E-05 | 0.1 | 20 | ||||||||
| 231 | Oxic Live | 24 | 0.0400 | 1.80E-04 | 10 | 1.35E-08 | 1.68E-05 | 0.1 | 1.10E-06 | 1.34E-05 | 1.23E-05 | 1.10E-08 | 20 | 3.25E-05 | 6.50E-07 | 6.58E-07 | 0.4 |
| 232 | Oxic Live | 24 | 0.0402 | 1.81E-04 | 10 | 1.35E-08 | 1.54E-05 | 0.1 | BDL | 1.59E-05 | 1.59E-05 | BDL | 20 | 2.50E-07 | 5.00E-09 | 5.00E-09 | 0.0 |
| 233 | Oxic Live | 24 | 0.0399 | 1.80E-04 | 10 | 1.35E-08 | 1.56E-05 | 0.1 | 20 | ||||||||
| 234 | Oxic Live | 48 | 0.0399 | 1.80E-04 | 10 | 1.35E-08 | 1.75E-06 | 0.0 | 20 | 2.50E-07 | 5.00E-09 | 5.00E-09 | 0.0 | ||||
| 235 | Oxic Live | 48 | 0.0401 | 1.80E-04 | 10 | 1.35E-08 | 1.60E-05 | 0.1 | BDL | 1.56E-05 | 1.56E-05 | BDL | 20 | 1.96E-05 | 3.92E-07 | 3.92E-07 | 0.2 |
| 236 | Oxic Live | 48 | 0.0399 | 1.80E-04 | 10 | 1.35E-08 | 1.59E-05 | 0.1 | 20 | ||||||||
| 237 | Oxic Live | 168 | 0.0399 | 1.80E-04 | 10 | 1.35E-08 | 1.86E-05 | 0.1 | 7.00E-07 | 1.64E-05 | 1.57E-05 | 7.00E-09 | 20 | 4.54E-05 | 9.08E-07 | 9.13E-07 | 0.5 |
| 238 | Oxic Live | 168 | 0.0399 | 1.80E-04 | 10 | 1.35E-08 | 1.887E-05 | 0.1 | 20 | 6.70E-06 | 1.34E-07 | 1.34E-07 | 0.1 | ||||
| 239 | Oxic Live | 168 | 0.0401 | 1.80E-04 | 10 | 1.35E-08 | 1.91E-05 | 0.1 | 20 | ||||||||
aBy ICP.
bBy ferrozine assay.
cIncludes 3 ml supernatant and 17 ml 0.6 N HC1.
dSum of Fe(II) in 0.5 N HC1 extraction solution and dissolved Fe(II) that was removed when 7 ml supernatant was removed.
eDetection limit of 0.4 μM was assumed after Washington et al. (Ref. 26).