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Growth comparison, expression profiles, and characterization of cytotoxicity associated with WT EHEC and its stx2 isogenic mutant. (A) Growth curves of strains 86-24 (□) and TUV86-2 (○) in Eagle’s minimal essential medium (EMEM) and LB. (B) Immunoblot evaluation of lysates of cultures of 86-24 or TUV86-2 for Stx2, intimin, and Tir. (C) Cytotoxic effects of serially diluted lysates of DH5α (▵), 86-24 (□), TUV86-2 (○), or medium (◇) alone on Vero cells after 48 h of incubation.
