Table 3.

Complementation of mutant myosin-II strains

Strain	Mediaa	Plasmidb	17°C	25°C	32°C	36°C
Δmyp2	EMM + 1 M KCl	pGFPLEU2	−	sg	sg	sg
Δmyp2	EMM + 1 M KCl	GFP-myp2	+	+	+	+
Δmyp2	EMM + 1 M KCl	GFP-myo2	−	−/+	sg	sg
Δmyp2	EMM + 1 M KCl	GFP-pHoT	−	sg	sg	sg
Δmyp2	EMM + 1 M KCl	GFP-oHpT	+	+	+	+
myo2-E1	YES	pGFPLEU2	ND	+	−	−
myo2-E1	YES	GFP-myp2	ND	+	−	−
myo2-E1	YES	GFP-myo2	ND	+	+	+
myo2-E1	YES	GFP-pHoT	ND	+	+	+
myo2-E1	YES	GFP-oHpT	ND	+	−	−
Δmyp2 myo2-E1	EMM	pGFPLEU2	ND	+	−	−
Δmyp2 myo2-E1	EMM	GFP-myp2	ND	+	−/+	−
Δmyp2 myo2-E1	EMM	GFP-myo2	ND	+	+	+
Δmyp2 myo2-E1	EMM	GFP-pHoT	ND	+	+	+
Δmyp2 myo2-E1	EMM	GFP-oHpT	ND	+	+/−	−
Δmyp2 myo2-E1	EMM + 1 M KCl	pGFPLEU2	−	sg	−	−
Δmyp2 myo2-E1	EMM + 1 M KCl	GFP-myp2	+	+	+	+
Δmyp2 myo2-E1	EMM + 1 M KCl	GFP-myo2	−	sg	−	−
Δmyp2 myo2-E1	EMM + 1 M KCl	GFP-pHoT	−	sg	−	−
Δmyp2 myo2-E1	EMM + 1 M KCl	GFP-oHpT	+	+	+	+
Δmyo2	EMM	pGFPLEU2	ND	ND	−	ND
Δmyo2	EMM	GFP-myp2	ND	ND	−	ND
Δmyo2	EMM	GFP-myo2	ND	ND	+	ND
Δmyo2	EMM	GFP-pHoT	ND	ND	+	ND
Δmyp2	EMM	GFP-oHpT	ND	ND	−	ND
Δmyp2 Δmyo2	EMM	pGFPLEU2	ND	ND	−	ND
Δmyp2 Δmyo2	EMM	GFP-myp2	ND	ND	−	ND
Δmyp2 Δmyo2	EMM	GFP-myo2	ND	ND	+	ND
Δmyp2 Δmyo2	EMM	GFP-pHoT	ND	ND	+	ND
Δmyp2 Δmyo2	EMM	GFP-oHpT	ND	ND	−	ND

pHoT denotes chimera with myp2⁺ head and myo2⁺ tail. oHpT denotes chimera with myo2⁺ head and myp2⁺ tail. +, colony formation; −, no colony formation; +/−, colonies of smaller size; −/+, microcolonies; sg, colonies form slower than wild type cells; ND, not determined. Complementation of Δmyo2 and Δmyo2 Δmyp2 accomplished by random spore analysis (see MATERIALS AND METHODS). 

^a YES is rich media. EMM is minimal media. 

^b All constructs under the control of the weakest nmt1⁺ promoter.