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. 2006 Mar 30;5:6. doi: 10.1186/1475-2840-5-6

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Copyright © 2006 Ehlermann et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Stimulation of AGE-pretreated (black squares) HUVEC with heterodimeric S100A8/S100A9 resulted in phosphorylation of both MAP kinases p38 (e) and Erk1/2 (k) within 15 minutes. Representative immunoblots for AGE preincubated HUVEC are shown for phosphorylated p38 (a), total p38 (b), phosphorylated Erk1/2 (f) and total Erk1/2 (g). Control experiments: Phosphorylated p38 (c), total p38 (d), phosphorylated Erk1/2 (h) and total Erk1/2 (i) *p < 0.05 AGE pretreatment vs. albumin-glucose (open circles) for control.