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. 2006 May 5;7:109. doi: 10.1186/1471-2164-7-109

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Copyright © 2006 Khan et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Signal intensity (log₂) of high (▲), medium (■) and low (●) mRNA abundances vs dilution. 1:25 dilution saturates high abundance RNA samples whereas 1:200 dilution results in undetectable signal for low abundance RNA. High, medium and low mRNA abundances. 1:50 dilution produces detectable but not saturated signal for the most spots.