Figure 5.

Validation of microarray result by simple detection analysis using RT-PCR for three candidate transcripts. Amplifications are performed in oocytes of two species with gene- and species-specific primers designed according to the known sequences in respective species. A) Amplification in mouse and Xenopus laevis oocytes of SMFN transcript originally found in the bovine oocyte-library. B) Amplification in bovine and Xenopus laevis oocytes of Spin transcript originally found in the mouse oocyte-library. C) Amplification in bovine and mouse oocytes of Prmt1 transcript originally found in the Xenopus laevis oocyte-library. Bt; Bos Taurus, Mm; Mus musculus, Xl; Xenopus laevis, Smfn; Small fragment nuclease, Spin; Spindlin, Prmt1; Protein arginine methyltransferase 1, Oo; oocyte, N; negative control.