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Comparison of all three recombinant pH-probes on a log-log scale. The areas of best responsiveness are highlighted by coloured rectangles: ecliptic pHluorin in red (emission ratio Rem = F511/F465 at λex = 400 nm); ratiometric pHluorin in green (excitation ratio Rex = F390/F475 at λem = 508 nm), and Pt-GFP in blue (excitation ratio Rex = F475/F390 at λem = 540 nm).
