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Arabidopsis line expressing Pt-GFP. A: Confocal image of Arabidopsis guard cells expressing Pt-GFP in the cytoplasm. The red fluorescence is chlorophyll autofluorescence from chloroplasts. B: Confocal optical sections and C: corresponding bright-field image of a root segment. Excitation with Argon laser line 476 nm; emission at 500–540 nm (green channel) and 600–660 nm (red channel); Leica TCS SP confocal laser scanning system; HC PL APD objective (40× oil).
