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. Author manuscript; available in PMC: 2006 Jun 12.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2004 Nov;45(11):3863–3870. doi: 10.1167/iovs.04-0590

FIGURE 3.

FIGURE 3

Ca2+-induced conformational changes in GCAP1 and its mutants observed by fluorescence spectroscopy. Changes in fluorescence at λex = 290 nm and λem = 343 nm as a function of [Ca2+]. Insets, fluorescence emission spectra of GCAPs using excitation at 280 nm from 4.6 × 10-8 M to 2.4 × 10-6 M Ca2+. (A) Recombinant human GCAP1; (B) GCAP1-E155G; (C) GCAP1-I143NT.