TABLE 1.
Primer | Exon | Primer Sequence | |
---|---|---|---|
GCAP1 | |||
4949 | 1S | 5′-GGACTCAGGCCTGTGAGAGA | |
4950 | 1A | 5′-CCAGCTGGTCAGGCTTCC | |
4951 | 2-4S | 5′-CGGAGCCTTGGGTTATGAT | |
4952 | 2-4A | 5′-GCGAGCTAAGCCTCTGAGTT | |
5094 | 2R* | 5′-CCTCAGTCCCACATCCTGTC | |
5095 | 3F* | 5′-TCTACTCCTCACTGCTCTTG | |
5097 | 4F* | 5′-ACGTGGGCTCTGTCCCTGCC | |
GCAP2 | |||
5098 | 1S | 5′-AGTCACCCAGGAGATGATGG | |
5099 | 1A | 5′-ACGGAGTGGAAAGAAGAGCA | |
5100 | 2S | 5′-GGCCACATGGATTAAAAGGA | |
5101 | 2A | 5′-CACAGGAAACACACTCAGAATGA | |
5102 | 3S | 5′-GTGATCCTAAGACGCACAGC | |
5103 | 3A | 5′-TTCTCCACAGCCTTCTCACA | |
5104 | 4S | 5′-TGAGGTCTGAGACTGCAGGA | |
5105 | 4A | 5′-GAAGTCAACACCAGGGGAAGA |
Exons terminating in an “S” are in the sense direction; those with “A” are in the antisense direction.
These primers were used for sequencing the PCR product amplified by 4951 and 4952.