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. 2000 Jan;11(1):103–116. doi: 10.1091/mbc.11.1.103

Figure 1.

Figure 1

(A) Multiple copies of TIM18 suppress the growth defect of tim54-1 mutants but not tim23-1 mutants. tim54-1 his3 strain 735 was transformed with one of the following high-copy plasmids: 2μ-TIM18 plasmid pOK66, 2μ-TIM54 plasmid pOK59, 2μ-TIM22 plasmid pJH203, or the empty vector pRS423 (Sikorski and Hieter, 1989). tim23-1 leu2 ura3 strain 574 was transformed with 2μ-TIM18 plasmid pOK66, 2μ-TIM23 plasmid pKR21 (Ryan et al., 1998), 2μ-TIM17 plasmid pKR7 (Ryan et al., 1994), or the empty vector pRS426 (Sikorski and Hieter, 1989). Transformants were streaked onto YEPmedium containing 2% glycerol and ethanol and incubated at 24 or 34°C for 5 d. (B) Multiple copies of TIM18 do not suppress the tim54::KAN or tim22::TRP1 disruptions. tim22::TRP1 strain 935, which carries the TIM22-URA3 plasmid pJH202 (Kerscher et al., 1997), was transformed with 2μ-TIM18 plasmid pOK66, 2μ-TIM22 plasmid pJH203, or the empty vector pRS423 (Sikorski and Hieter, 1989). tim54::KAN strain 1078, which carries the TIM54-URA3 plasmid pOK30 (Kerscher et al., 1997), was transformed with 2μ-TIM18 plasmid pOK66, CEN-TIM54 plasmid pOK22 (Kerscher et al., 1997), or the empty vector pRS424 (Sikorski and Hieter, 1989). Transformants were patched onto YEPD medium and then replica plated onto medium containing 5-FOA to select for loss of the TIM22-URA3 or TIM54-URA3 plasmids.