Figure 4.

Indirect immunofluorescence analysis of wild-type, pex23 mutant, and PEX23-transformed strains. Wild-type (WT) strain E122, mutant strains pex23-1 and pex23KOA, and transformed strain P23TR were grown in YPBO medium (YNO medium for strain P23TR). Cells were processed for immunofluorescence microscopy with antibodies to the PTS1 tripeptide SKL (SKL), thiolase (THI), isocitrate lyase (ICL), and acyl-CoA oxidase (AOX). Rabbit primary antibodies (SKL, ICL, AOX) were detected with fluorescein-conjugated goat anti-rabbit immunoglobulin G secondary antibodies, and guinea pig primary antibodies (THI) were detected with rhodamine-conjugated donkey anti-guinea pig immunoglobulin G secondary antibodies.