Figure 2.

Specificity of NF-κB activation. (A) Activation of NF-κB-dependent luciferase transcription in Mv1Lu cells incubated 2 h with Silvio strain trypomastigotes or epimastigotes at a concentration of 1 × 10⁷/ml in RPMI and 1% BSA. Cells were harvested 24 h after addition of parasites. Results represent the mean of triplicate transfections ± SEM. (B) Nuclear translocation of p65 in Mv1Lu cells exposed for 1 h to RPMI and 1% BSA and Silvio, Tulahuen, or MV13 strain parasites at 1 × 10⁷/ml in RPMI and 1% BSA. Results represent the mean of duplicate assays ± range.