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Preincubation experiment to test absorption of motors to particles. Gold particles with TUB 2.1 anti-β-tubulin antibody were preincubated 45 min in a kinesin-coated chamber, then transferred to a second chamber coated with 14S dynein. (A) Tracking curves for MT ends and attached particle in the dynein-coated chamber. (B) Velocity profile. The particle moved toward the trailing (−) end of the MT.
