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ER to Golgi transport assay results in both α1,6-mannosylated and α1,2-mannosylated forms of pro-αf. Semi-intact yeast cells were incubated with cytosol, 35S-labeled prepro-αf, and ATP for the times indicated. Samples from each time point were removed and immunoprecipitated with either α1,6-linkage serum (A) or concanavalin A-Sepharose (B), split evenly, and treated with or without endoM.
