TABLE 2.
TRANSDUCED TIL SPECIFICITY
| Stimulators |
|||||
|---|---|---|---|---|---|
| Responder cells | Vector | T2 alone | T2+FLU | T2+gp100 | T2+MART |
| pg/ml IFN-γ | |||||
| TIL | none | 73 | 59 | 64 | 58 |
| TIL | MSGIN | 451 | 242 | 309 | 301 |
| APB 9 | gp100 | 95 | 63 | 11,345 | 100 |
| AIB 18 | MART | 119 | 90 | 95 | 14,695 |
| JB2F4 | none | 1 | 0 | 50 | 20,800 |
| RC612 | none | 0 | 12 | 28,350 | 15 |
| pg/ml GM-CSF | |||||
| TIL | none | 122 | 97 | 98 | 104 |
| TIL | MSGIN | 1820 | 1464 | 1833 | 1526 |
| APB 9 | gp100 | 502 | 409 | 36,329 | 532 |
| AIB 18 | MART | 333 | 242 | 297 | 79,960 |
| JB2F4 | none | 14 | 13 | 162 | 8042 |
| RC612 | none | 25 | 170 | 1400 | 226 |
| pg/ml TNF-α | |||||
| TIL | none | 15 | 7 | 9 | 10 |
| TIL | MSGIN | 21 | 27 | 21 | 16 |
| AIB18 | MART | 6 | 306 | 518 | 1,568 |
| JB2F4 | none | 3 | 508 | 896 | 2,835 |
Cytokine production from coculture of transduced TIL and peptide pulsed T2 cells. MSGIN is a control vector containing GFP. APB 9 is a retroviral vector encoding the anti-gp100 TCR. AIB 18 encodes the anti-MART-1 TCR. JB2F4 and RC612 are cytotoxic T lymphocytes, which are highly MART-1 and gp100 reactive, respectively.
TIL, tumor-infiltrating lymphocytes; IFN-γ, interferon-γ; TNF-α, tumor necrosis factor-α; IL; GM-CSF, granulocyte macrophage-colony stimulating factor.