TABLE 3.
TRANSDUCED TIL MELANOMA REACTIVITY
| Stimulators |
|||||
|---|---|---|---|---|---|
| Responder | Vector | MEL 526 | MEL 624 | MEL 888 | MEL 938 |
| pg/ml IFN-γ | |||||
| TIL | none | 22 | 22 | 24 | 42 |
| TIL | MSGIN | 14 | 13 | 58 | 13 |
| AIB 18 | MART | 2139 | 1674 | 18 | 14 |
| APB9 | gp100 | 364 | 176 | 8 | 0 |
| JB2F4 | none | 4900 | 8100 | 0 | 1 |
| RC612 | none | 11,285 | 6650 | 0 | 0 |
| pg/ml GM-CSF | |||||
| TIL | none | 34 | 32 | 44 | 84 |
| TIL | MSGIN | 29 | 35 | 437 | 122 |
| AIB 18 | MART | 2802 | 3125 | 51 | 105 |
| APB 9 | gp100 | 2850 | 1057 | 101 | 136 |
| JB2F4 | none | 4365 | 3961 | 3 | 86 |
| RC612 | none | 1448 | 1464 | 8 | 84 |
Rescue of nonreactive TIL cultures. Cytokine production from coculture of transduced TIL and melanoma cell lines. Untransduced TIL or TIL transduced with GFP control vector (MSGIN), anti-MART-1 TCR vector (AIB18), or anti-gp100 TCR vector (APB9) were cocultured with the indicated cell lines (stimulators). MEL 526 and 624 are HLA-A2-positive melanoma lines while MEL 888 and 938 are A2-negative. JB2F4 and RC612 are cytotoxic T lymphocytes, which are MART-1 and gp 100 reactive, respectively.
TIL, tumor infiltrating lymphocytes; GFP, green fluorescent protein; IFN-γ, interferon-γ; GM-CSF, granulocyte macrophage-colony stimulating factor.