Figure 2.

A time course of butanol-induced morphological changes in liquid medium. Strains MLY41 (wild-type MATa), MLY61 (wild-type MATa/α), and 27-06 (Δste12 MATa) were grown overnight in liquid YPD medium, diluted into 5 ml of YPD with (+) or without (−) 1% (vol/vol) butanol in 15-ml sealed, screw-cap tubes, and grown at 30°C for the indicated times. Cells were spotted to microscope slides and photographed at ×400 magnification. The images at the 30-h time point are presented at half the magnification of the other panels to show the multicellular asters that develop in the haploid wild-type strain.