Table 2.

Oligonucleotide primers used to create disruption strains

Strain	Gene	Primer	Sequence
SCY40	MRP21	5′ disruption	ATGTTGAAGAGCACGCTGAGGCTTTCAAGAATCTCTCTCACAGCTGAAGCTTCGTACGC
		3′ disruption	TCTTGAAGCCCATCATGAATTCCTTCCTATGCCTTTGAGAGCATAGGCCACTAGTGGATCTG
		3′ check	GTATCCTTTCCTCTTGGCATC
SCY41	YOL095	5′ disruption	ATGGACAAGCTAACTCCATCTCAATGGAAGGTAATAAATAACAGCTGAAGCTTCGTACGC
		3′ disruption	CTTAGTGAAGAATATGCTCTATAAAATCCAAAATTTTTGCATAGGCCACTAGTGGATCTG
		3′ check	CTATATACGTCTGAAAACGC
SCY42	MSM1	5′ disruption	ATGCAATGTCGATCAATTGTGCATCGTCTGTACTCTAAGGTCAGCTGAAGCTTCGTACGC
		3′ disruption	ATGGAATTTTTTTCAAAGGTACTTCTCGGCCTTTCTTGGCATAGGCCACTAGTGGATCTG
		3′ check	TCATATTCGTTTGTTCCTCT
SCY44	FIG1	5′ disruption	ATGGTCGCAATCTCAATGATTTGGTTTTTTACCAAGCGTATCAGCTGAAGCTTCGTACGC
		3′ disruption	ACATTATCAATTTCATCTTTCAAGCTTTTCCTATCCCTGCATAGGCCACTAGTGGATCTG
		3′ check	ATGTAGATGAATCCGAAGAG
SCY45	BEM4	5′ disruption	TGGATTACGAAGAAATTTTATTTGGTCTGCAACCGATCCTCCAGCTGAAGCTTCGTACGC
		3′ disruption	TAAATTTCCCATTGTTGAAACAACTCTCGCTACCCTTAGCATAGGCCACTAGTGGATCTG
		3′ check	TTGTTCCTCTGGCGTTAAGC
SCY46	BEM1	5′ disruption	ACTTCAAACTCTCAAAAAGAGATAGTAATGGGTCGAAGGGCCAGCTGAAGCTTCGTACGC
		3′ disruption	TCCAGAAGTTGATTGGGCTGGAGCCTGCTTGGAGCCCGGCATAGGCCACTAGTGGATCTG
		3′ check	GAAATTTTCAGTTTGGCTTGG
MLY261	STE4	5′ disruption	GAAAATACTCAAAAAACTGTACAGCTCAATCAGGTACACATTACGCAGCTGAAGCTTCGTACGC
		3′ disruption	CACAGTATTTCCAATTCGAAGCTATTGATAACCTGGAGACCATATGCATAGGCCACTAGTGGATCTG
		5′ check	GAATCCCTTCAATATCGAAAC