Figure 2.

Northern blots of poly(A)⁺ RNA (5 μg/lane) isolated from Chlamydomonas before and after (30 min) deflagellation. (A) pBrib43a hybridizes with a 1.7-kb band, which is up-regulated after deflagellation. (B) pCRY1-1, a clone coding for the S14 ribosomal protein, which is not involved in flagellar assembly (Nelson et al., 1994), hybridizes with 0.9-kb bands of equal intensity before and after deflagellation. These results demonstrate that the up-regulation seen with pBrib43a is not due to unequal loading of RNA.