Figure 4.

Predicted sequence of rib43a and comparison with other sequences in the database. The largest open reading frame of the pBrib43a cDNA consists of 1612 bp encoding rib43a, the 367-amino-acid protein from Chlamydomonas ribbons with a molecular weight of 42,638. The predicted sequence of rib43a is compared with sequences (ESTs) from T. cruzi and tumors of H. sapiens. Compared with rib43a, identical and conservatively substituted residues are shown in black and gray, respectively. Between rib43a and the Trypanosoma EST there is 35% identity and 46% similarity (similarity = identities + conservative substitutions) with no gaps in the sequences; between rib43a and the human tumor ESTs these values are 28 and 37% for EST1 and 26 and 38% for EST2 (with only one gap). Several blocks of amino acid residues are identically conserved between rib43a and the trypanosome EST (e.g., RVGDDD) and between rib43a and both of the human tumor ESTs (e.g., KGMT). Note that rib43a has no cysteine residues. The references of the clones are as follows: rib43a (this report; GenBank accession numbers AF196576 for the cDNA clone and AF196577 for the genomic clone); Trypanosome (GenBank accession number AI080816); human EST1 (GenBank accession number AI890123) from moderately differentiated, endometrial adenocarcinoma, three pooled tumors; and human EST2 (GenBank accession number AI671905) from pooled germ cell tumors.