Figure 1.

Characterization of human VPS4. The amino acid sequence of human VPS4 was compared with those of human and mouse SKD1 and yeast Vps4p. Identical amino acids are outlined in black. Those amino acids whose identity is conserved only between three proteins are outlined in dark gray. Light gray indicates residues where identity is limited to two proteins. The location of the N-terminal coiled coil–forming region that was deleted for some experiments is indicated by a double overline, and that of the AAA domain is indicated by a single line. Point mutations used in this study are also shown. The sequence data for hVPS4 are available from GenBank under accession number AF038960.