Figure 5.

Expression of hVPS4(EQ) gives rise to aberrant early endosomes. BHK cells were transfected with GFP-hVPS4(EQ) (A–C) or GFP-hVPS4(wt) (D–F) and visualized directly (A and D; green) or after incubation with anti-TfR (B and E; red). Merged images are shown (C and F). Arrows (A and B) provide examples of where GFP-hVPS4(EQ) and TfR colocalize. Note the change in TfR distribution in cells transfected with GFP-hVPS4(EQ) compared with GFP-hVPS4(wt) or untransfected cells. Cells in A–C were permeabilized with saponin before fixation.