Figure 1.

Spindle pole movements in wild-type embryos analyzed in real time. (A) Plots of spindle pole separation versus time during interphase–prophase of cycle 13 (top panel), prometaphase–metaphase of cycle 12 (middle panel), and anaphase B of cycle 12 (bottom panel) in control-injected wild-type Drosophila early embryos. Measurements are from time-lapse confocal micrographs taken every 5 s from living Drosophila embryos injected with fluorescent tubulin. The star in the middle panel marks the onset of metaphase. (B–E) Still images showing tubulin fluorescence at the time points marked B–E on the plots shown in A. Bar, 7.5 μm. Video for B–E, Mitotic cycles 11–13 in wild-type Drosophila embryos: time series of confocal micrographs from a living Drosophila embryo injected with fluorescent tubulin. The number of the occurring mitotic cycle is indicated in the top right corner of the video. Actual elapsed time, 25 min 55 s.