Figure 6.

Inhibition of autophagy by 3-MA results in the disappearance of swollen LAMP-2-positive MLBs. Untransfected Mv1Lu (A and B) and GlcNAc-TV-transfected M9 (C and D) cells were incubated in regular medium (A and C) or medium supplemented with 10 mM 3-MA for 3 d (B and D) and then immunofluorescently labeled for LAMP-2. Although 3-MA treatment induces the formation of slightly swollen lysosomal structures in both Mv1Lu and M9 cells (B and D), it also results in the disappearance of the large LAMP-2-positive vacuoles, which correspond to MLBs in M9 cells (D). Bar, 10 μm.