Table 1.
Quantification of MLB expression in GlcNAc-TV-transfected Mv1Lu cells
| Cell type | GlcNAc-TV (pmol/mg/h)a | Total no. of MLBs | Range of MLB size (μm2) | Total cytoplasmic area (μm2) | Total MLB area (μm2) | Area MLB/cytoplasm (%) |
|---|---|---|---|---|---|---|
| Mv1Lu | 63 ± 6 | 1 | 0.53–0.53 | 2022 | 0.53 | 0.03 ± 0.03 |
| C1 | 59 ± 5 | 29 | 0.27–2.00 | 1875 | 22.5 | 1.1 ± 0.6 |
| R2 | 151 ± 15 | 15 | 0.33–1.60 | 1882 | 12.3 | 0.6 ± 0.3 |
| M9 | 567 ± 265 | 78 | 0.23–16.3 | 1690 | 223 | 13.0 ± 1.1 |
| M1 | 1082 ± 308 | 98 | 0.22–14.0 | 1821 | 109 | 6.1 ± 1.6 |
From 10 electron microscopy images (4400×) of each cell line, the area covered by MLBs and by cytoplasm (excluding the nucleus) in the complete image was determined. Using a two-tailed Student's t test, the ratio of MLB to cytoplasmic area in M9 cells is significantly different from both Mv1Lu and C1 cells (p < 10−5). For M1 cells, the ratio of MLB to cytoplasmic area is also significantly different from both Mv1Lu (p < 0.005) and C1 (p < 0.01) cells. The significance of the difference in MLB/cytoplasm area between either C1 or R2 cells and Mv1Lu cells is of the order of 0.05 < p < 0.1.
a
The values for GlcNAc-TV expression were taken from Dimetriou et al. (1995).