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. 2000 Jan;11(1):287–304. doi: 10.1091/mbc.11.1.287

Figure 4.

Figure 4

Figure 4

Ultrastructural analysis of Rac1V12 cells grown in the presence or absence of DC. Fab-HRP was internalized from the basolateral pole of the cell, the cells were fixed, a DAB reaction was performed, and the cells were processed for electron microscopy. (A) Cells grown in the presence of DC. (B) Cells grown in the absence of DC. Clusters of Fab-HRP–labeled structures are labeled with asterisks. The upper cluster represents ligand present in the common endosome/ARE, and the bottom cluster represents ligand present in the central aggregate. (Inset) A magnified view of the endosomal elements that constitute the central aggregate. Ct, centriole. (C) Cells grown in the absence of DC. A juxtanuclear central aggregate is shown. (Inset) A magnified view of the 10-nm filaments surrounding the endosomal elements of the central aggregate (marked with arrows). G, Golgi stacks; LF, infoldings of the lateral membrane; Nu, nucleus.