Figure 4.

Invertase is fully glycosylated and secreted from vps52Δ and triple mutant strains. Wild-type (SNY36-9B), vps52Δ::kan^r (LCY222), and vps52Δ::kan^r vps53::Tn3URA vps54Δ::TRP1 (LCY280) strains containing a plasmid encoding SUC2 (pFvM104) were grown in low-glucose medium for 45 min to induce invertase expression before labeling with [³⁵S]methionine for 7 min and chasing for 30 min. Invertase was immunoprecipitated from intracellular (I) and extracellular (E) fractions and analyzed by SDS-PAGE and fluorography.