Table 1.
Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pLC63 | CEN-LEU2 plasmid encoding Vps53p | This study |
| pLC64 | CEN-LEU2 plasmid encoding Vps52p | This study |
| pLC65 | CEN-LEU2 plasmid encoding Vps52p-myc | This study |
| pLC66 | CEN-URA3 plasmid encoding Vps54p | This study |
| pLC68 | CEN-LEU2 plasmid encoding Vps53p-myc | This study |
| pLC72 | CEN-LEU2 plasmid encoding Vps52p-HA | This study |
| pLC75 | CEN-LEU2 plasmid encoding Vps53p-HA | This study |
| pLC103 | CEN-URA3 plasmid encoding Vps54p-myc | This study |
| pLC104 | CEN-URA3 plasmid encoding Vps54p-HA | This study |
| pLC89 | Integrating plasmid for VPS52-myc (pRS303 based) | This study |
| pLC91 | Integrating plasmid for VPS53-myc (pRS304 based) | This study |
| pLC92 | Integrating plasmid for VPS53-HA (pRS304 based) | This study |
| pLC95 | CEN-URA3 plasmid encoding Vps52p-HA | This study |
| pSN55 | CEN-URA3 plasmid encoding A-ALP | (Nothwehr et al., 1993) |
| pSN92 | CEN-URA3 plasmid encoding ALP | (Nothwehr et al., 1993) |
| pNB6 | CEN-LEU2 plasmid encoding ALP | T.H. Stevens |
| pSN246 | CEN-LEU2 plasmid encoding A-ALP | (Nothwehr et al., 1996) |
| pFvM104 | CEN-URA3 plasmid encoding invertase | (Fischer von Mollard et al., 1997) |
| pLC115 | Integrating plasmid for Vps10p-HA | This study |
| pLC116 | Integrating plasmid for Och1p-HA | This study |
| pSLA2 | CEN-URA3 plasmid encoding End4p | D. Drubin |
| pSN273 | Plasmid containing pep4Δ∷LEU2 disruption cassette | (Nothwehr et al., 1996) |
| pLC28 | Plasmid for making vps53∷Tn3∶LacZ-URA3 disruption | This study |
| pLC121 | Plasmid for making vps52∷Tn3∶LacZ-URA3 disruption | This study |
| pRSQ306 | Derivative of pRS306 containing part of LEU2 | J. Horecka |
| pRSQ303 | Derivative of pRS303 containing part of LEU2 | J. Horecka |