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. 2000 Jan;11(1):339–354. doi: 10.1091/mbc.11.1.339

Figure 2.

Figure 2

Space-filling model of human Cdc42p (GDP bound) shown in three different orientations (Brookhaven Protein Database accession number 1AN0). Colored residues correspond to an equivalent S. cerevisiae Cdc42p residue that was mutated in this study (see Figure 1A). When integrated into the S. cerevisiae genome, the mutations conferred lethality (red residues), temperature sensitivity (orange residues), cold sensitivity (blue residues), or both cold and temperature sensitivity (pink residues) to cells grown on rich medium. Mutations that did not confer a growth defect are colored green. GDP is colored gray. Some mutated residues are indicated by the corresponding allele number. (A) Face of Cdc42p that contains the switch I and switch II regions. (B) A 180° rotation of Cdc42p shown in A around the vertical axis. A putative intermolecular contact region is circled. (C) A left 90° rotation of Cdc42p shown in A around the vertical axis. The spatial separation of the putative intermolecular contact region shown in B from switch I is shown. The two regions are separated by a meridian of uncharged residues (uncolored).