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(A) Indirect immunofluorescence of wild-type (CDC42; DDY1300) and mutant (cdc42-1; DDY1302) S. cerevisiae grown at 25°C in rich medium and probed with affinity-purified anti-Cdc42 peptide antibody. Bar, 10 μm. (B) Indirect immunofluorescence of S. cerevisiae cells, from the same cultures described in Figure 3C, probed with affinity-purified anti-Cdc42 peptide antibody. The scale is the same as in A.
