Table 2.

Determination of terminal phenotypes of cdc42^ts strains at 37°C

	Hours at 37°C	Percent unbudded	Percent small budded	Percent medium budded	Percent large budded	Percent other
CDC42	0	42	30	8	19	0
	6	46	33	6	16	0
cdc42-1	0	62	30	4	5	0
	6	89	8	<1	1	2
cdc42-101	0	46	30	10	15	0
	6	97	2	1	<1	0
cdc42-118	0	56	27	2	15	0
	6	91	4	<1	4	1
cdc42-123	0	75	18	3	5	0
	6	88	4	1	2	6
cdc42-124	0	58	30	3	9	<1
	6	78	11	1	3	8
cdc42-129	0	52	23	3	3	19
	6	39	4	2	1	55

For each time point, 200 cells were scored by phase-contrast microscopy. A cell was scored as unbudded if no bud was observed, small budded if the volume of the bud appeared <30% the volume of the mother cell, medium budded if the volume of the bud appeared 30–50% the volume of the mother cell, and large budded if the volume of the bud appeared >50% the volume of the mother cell. “Other” describes grossly misshapen buds and cells, including elongate buds.