Table 4.
Suppression of cdc42ts growth defect by effector overexpression
| Vector | STE20 | CLA4 | SKM1 | GIC1 | GIC2 | |
|---|---|---|---|---|---|---|
| CDC42 | + | + | − | + | + | + |
| cdc42-101 | − | − | − | − | + | − |
| cdc42-118 | − | + | − | − | − | − |
| cdc42-123 | − | +/− | − | − | +/− | +/− |
| cdc42-129 | − | − | +/− | +/− | − | − |
| cdc42-1 | − | +/− | − | − | +/− | − |
cdc42ts strains (MATa; see Table 1) were transformed with galactose-inducible URA3-marked cen plasmids containing known effectors of Cdc42. Transformation with vector (pRB1438), pGAL-STE20, pGAL-CLA4, pGAL-SKM1, pGAL-GIC1, and pGAL-GIC2 yielded, respectively, CDC42: DDY 1610, 1611, 1612, 1613, 1614, 1615; cdc42-1: DDY 1616, 1617, 1618, 1619, 1620, 1621; cdc42-101: DDY 1622, 1623, 1624, 1625, 1626, 1627; cdc42-118: DDY 1628, 1629, 1630, 1631, 1632, 1633; cdc42-123: DDY 1634, 1635, 1636, 1637, 1638, 1639; and cdc42-129: DDY 1640, 1641, 1642, 1643, 1644, 1645. Transformants were struck to single colonies on selective medium containing galactose and incubated at 37°C. + indicates wild type growth, +/− indicates weak growth, and − indicates no growth (single colonies). Overexpression of CLA4 inhibits the growth of wild-type cells at 37°C but not 25°C. cdc42-124 was not included in this analysis because it is not temperature sensitive on selective media.