Table 2.
Effects on nuclear export and stability of various mRNA species in stage IV oocytes assayed after over-expression or knockdown of Xp54 or over-expression of a DEAD-box mutant
| mRNA | Translated | EFFECT ON mRNA EXPORT | EFFECT ON mRNA STABILITY | ||||
|---|---|---|---|---|---|---|---|
| Over-expression of | Injection of | Over-expression of | Injection of | ||||
| Xp54-wt | Xp54-DQAD | Antisense MO | Xp54-wt | Xp54-DQAD | Antisense MO | ||
| HistoneB4 | Embryo | 0 | 0 | 0 | 0 | 0 | 0 |
| FRGY1 | Embryo | 0 | − | − | + | − | − |
| Cyclin B1 | Maturation | 0 | − | − | + | − | 0 |
| HistoneH4 | Oocyte | 0 | 0 | 0 | − | 0 | + |
| rp L1 | Oocyte | + | − | 0 | 0 | + | + |
| rp S1 | Oocyte | + | − | − | 0 | + | + |
Nuclei and cytoplasms were isolated at 0 and 24 h after injection of wild-type (wt) or mutant (DQAD) expression vectors or of antisense or control morpholinos (MO). Steady-state levels of specific mRNAs were measured in all samples by semi-quantitative RT–PCR. Effects on mRNA export were calculated from changes in nuclear:cytoplasmic ratios and effects on mRNA stability were calculated from changes in total oocyte amounts. Changes are indicated as significant increase (+), significant decrease (−), insignificant change (0), where significance is set a >2-fold difference. Data were collected from three separate experiments.