Table 2.
mRNA | Translated | EFFECT ON mRNA EXPORT | EFFECT ON mRNA STABILITY | ||||
---|---|---|---|---|---|---|---|
Over-expression of | Injection of | Over-expression of | Injection of | ||||
Xp54-wt | Xp54-DQAD | Antisense MO | Xp54-wt | Xp54-DQAD | Antisense MO | ||
HistoneB4 | Embryo | 0 | 0 | 0 | 0 | 0 | 0 |
FRGY1 | Embryo | 0 | − | − | + | − | − |
Cyclin B1 | Maturation | 0 | − | − | + | − | 0 |
HistoneH4 | Oocyte | 0 | 0 | 0 | − | 0 | + |
rp L1 | Oocyte | + | − | 0 | 0 | + | + |
rp S1 | Oocyte | + | − | − | 0 | + | + |
Nuclei and cytoplasms were isolated at 0 and 24 h after injection of wild-type (wt) or mutant (DQAD) expression vectors or of antisense or control morpholinos (MO). Steady-state levels of specific mRNAs were measured in all samples by semi-quantitative RT–PCR. Effects on mRNA export were calculated from changes in nuclear:cytoplasmic ratios and effects on mRNA stability were calculated from changes in total oocyte amounts. Changes are indicated as significant increase (+), significant decrease (−), insignificant change (0), where significance is set a >2-fold difference. Data were collected from three separate experiments.