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Toxofilin localizes at one end of actin filaments. Actin filaments at steady state (2 μM) were incubated with purified r-Toxofilin (1 μM). Immunolocalization of r-Toxofilin was revealed by anti-rabbit antibodies conjugated to 5-nm gold (see arrows). Actin filaments were negatively stained with 2% uranyl acetate.
